Please use this identifier to cite or link to this item: https://dspace.ctu.edu.vn/jspui/handle/123456789/69648
Title: A comparison between Hydrochloric acid and Trifluoroacetic acid in hydrolysis method of exopolysaccharide from Ophiocordvceps sinensis in Monosaccharide composition analysis by GC-FID
Authors: Le, Thi Thuy Hang
Ha, Bao Chau
Tran, Van Hai Nam
Phan, Minh Thong
Dang, Hoang Phu
Dinh, Minh Hiep
Nguyen, Tien Thang
Keywords: EPS hydrolysis
GC-FID
HPLC
Monosaccharide
Issue Date: 2019
Series/Report no.: Journal of Science Ho Chi Minh Open University;Vol. 10, No. 01 .- P.22-28
Abstract: The monosaccharide composition is one of the crucial factors affecting the bioactivity of exopolysaccharide (EPS) in Cordyceps species. Therefore, many scientists have studied, analyzed monosaccharide composition and structure of EPS from Cordyceps species, especially Ophiocordyceps sinensis (O. Sinensis). This study aimed to compare hydrochloric acid (HC1) with trifluoroacetic acid (TFA) in the EPS hydrolysis process in monosaccharide composition analysis by Gas Chromatography with Flame-Ionization Detection (GC-FID). The hydrolysis is a crucial step in forming the acetyl derivative, which helps the GC-FID technique to have good results in monosaccharide composition analysis. The results showed that hydrolysis with HC1 gave a higher hydrolysis efficiency and was more suitable than hydrolysis by TFA in pretreatment to EPS for GC-FID. Hydrolysis results were analyzed through thin-layer chromatography and high-performance liquid chromatography (HPLC), then Acetyl derivatives were produced and finally analyzed by GC-FID to determine the monosaccharide composition of EPS. For EPS hydrolyzed by HC1, the analytical results presented that this sample had 6 kinds of monosaccharides, including rhamnose, arabinose, xylose, mannose, glucose, and galactose; the most monosaccharide was glucose. The EPS hydrolyzed by TFA only detected three kinds of monosaccharides, including mannose, arabinosc, and galactose, mainly mannose. The study has set a foundation for further analysis of monosaccharide composition and structure of EPS from o. sinensis.
URI: https://dspace.ctu.edu.vn/jspui/handle/123456789/69648
ISSN: 1859-3453
Appears in Collections:Khoa học Trường ĐH Mở Tp.HCM (Journal of Science HCM Open University)

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