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Title: Inducer-free over expression oe beta-galactosidase in bacillus subtilis by using promoter PGRAC57
Authors: Tran, Thi Minh Dinh
Le, Thi Phuong Ngan
Phan, Thi Phuong Trang
Nguyen, Duc Hoang
Keywords: Bacillus subtilis
Inducer-free vector
Pgrac57 promoter
pHT expression vectors
Issue Date: 2019
Series/Report no.: Journal of Biotechnology;№ 17(02) .- Page.377-386
Abstract: A thermostable (β-galactosidase (BgaB) from Bacillus stearothermophilus is a potential candidate to be exploited in the dairv industry thank to its performance of high-level hydrolysis of lactose at high temperature. Production of BgaB in a mesophilic host using recombinant protein techniques is an effective method in the industrial scale. Bacillus subtilis is an ideal host cell factory for its well-known advantages. Inducer-free pHT plasmids based on Pgrac 01 and Pgrac 100 were previously reported to express reporter proteins at high levels in the absence of inducers.
ISSN: 1811-4989
Appears in Collections:Công nghệ sinh học

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