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Title: Degradation of 2,4-dichlorophenoxyacetic acid by Pseudomonas fluorescens strain HH
Authors: Nguyen, Thi Oanh
Ha, Danh Duc
Tran, Dat Huy
Nguyen, Gia Hien
Nguyen, Thi Huynh Nhu
Keywords: Pseudomonas fluorescens HH
2,4-Dichlorophenoxyacetic acid
Edwards model
Maximal utilization rates
Inhibition coefficient
Issue Date: 2018
Series/Report no.: Academia journal of biology;Vol. 40, No. 03 .- P.65-73
Abstract: Pseudomonas fluorescens HH isolated from soil utilized 2,4-Dichlorophenoxyacetic acid (2,4D) as a sole carbon and energy source. The strain completely utilized 1.0 mM of 2,4D within 30 hr. The immobilized Pseudomonas fluorescens HH in alginate degraded 2,4D with higher rates compared to the rates of free-suspension cells. The determination of depradation and cell growth kinetics in exponential growth phase of bacteria showed that both fitted with the Edwards model, ¡in which the maximal utilization rates and inhibition coefficient were 0.079 ± 0.008 mM/h and 0.820±0.03 mM, respectively. The addition of glycerol as a cryoprotectant into alginate increased the survival of bacteria in beads during freeze-drying process, which resulted in reducing the adverse effects of bead lyophilization.
ISSN: 2615-9023
Appears in Collections:Academia journal of biology

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